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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be observed in several tumors, which ends up in chromosome instability, and thus may add to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a essential role in endorsing mobile-cycle development during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, have already been explained. Two supplemental splice variants have been claimed but in-depth nucleotide sequence information is not but accessible.
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Blocking peptides are peptides that bind exclusively for the goal antibody and block antibody binding. These peptide typically includes the epitope identified by the antibody.
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This guide outlines further achievable optimization of mobile and immunoassay parameters to make sure the best possible results are...
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This guideline outlines further more probable optimization of cellular and immunoassay parameters to ensure the absolute best benefits are...
Antibodies sure to the blocking peptide no longer bind for the epitope to the concentrate on protein. By evaluating the staining from your blocked antibody as opposed to the antibody alone, one can see which staining is certain.
Target Data Cyclin E belongs towards the highly conserved cyclin spouse and children, whose members are characterised by a extraordinary periodicity in protein abundance through the mobile cycle. Cyclins perform as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation patterns which contribute to your temporal coordination of each mitotic celebration. Cyclin E forms a complex with and functions being a regulatory subunit of CDK2, whose exercise is required for cell cycle G1/S transition. This protein accumulates with the G1-S period boundary and is also degraded as cells progress through S stage.
The measurement of protein phosphorylation is a useful get more info tool for measuring the modulation of receptor activation by both antibodies...
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